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B12 injections are indicated for patients with documented deficiency, including those with pernicious anaemia, malabsorption disorders, following gastric surgery, or with neurological symptoms suggesting B12 deficiency

Enzymes were extracted using 100 mM phosphate buffer (pH 6.8) containing KH 2 PO 4 and K 2 HPO 4

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A five-month study in mice administering 20% D-mannose via drinking water reported no adverse effects on body weight, organ function, metabolism, or reproductive parameters, suggesting good tolerance even at high doses 26